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KMID : 0603519990040010025
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Journal of Korean Association of Cancer Prevention
1999 Volume.4 No. 1 p.25 ~ p.33
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DNA Ploidy Patterns in Mammary Gland Tumors Induced by 7,12-dimethylbenz[a]anthracene (DMBA) in Rats
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Kang Jin-Seok
Ahn Byeong-Woo
Kim Dae-Joong
Kim Ki-Sok
Nam Ki-Taek
Han Beom-Seok
Bae Jong-Hee
Bak Ung-Bok
Jang Dong-Deuk
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Abstract
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DNA cytometry has frequently been used to characterize DNA ploidy pattern in relation to clinical and histopathological findings. Anueploidy of tumor cells has been considered to be related with poor prognosis of patients. This study was carried out to examine the relationship between DNA ploidy patterns obtained by flow cytometry (FCM) & static cytometry (SCM) and histopathological observations of mammary gland tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) in rats. Eighteen from the total of 40 SD rats treated with DMBA (10 mg/kg) developed 39 tumor masses. Tumor incidence was 45.0% and tumor multiplicity was 0.97. Adenocarcinoma was most predominant subtype among DMBA-induced tumors. In 35 cases of malignant tumors, the incidence of aneuploid tumor was slightly higher in SCM than in FCM. In comparison of histopathological findings with DNA ploidy patterns aneuploidy was correlated with the grade of mitotic rate in malignant tumors (P<0.05). Morphological parameters such as nuclear areas and perimeter of nuclei were increased tendency with aneuploidy. Our results suggest that increased tendency of malignancy might be correlated with the degree of aneuploidy in mammary gland tumors of rats.
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KEYWORD
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Rat, DMBA, Mammary tumor, Histopathological observation, DNA ploidy
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